Calendar


SES # TOPICS KEY DATES
1 Introduction to the problem Assignment 1 out
2

Side-chain review

Sulfur redox chemistry

3 The Anfinsen experiments Assignment 2 out
4

Globular protein structure

Protein interiors

5 Using the Protein Database (PDB) Assignment 3 out
6 Helix-helix packing in globular proteins
7

Beta-sheets

Beta-sheet packing

8 Experimental techniques: circular dichroism Assignment 4 out
9

Thermal denaturation

Coiled coils

Refolding of Tropomyosin

10 Fluorescence spectroscopy Assignment 5 out
11 S-peptide helical folding
12 Detecting partially folded intermediates
13 Prolyl isomerization Assignment 6 out
14 Cytochrome c refolding pathway
15 2-D NMR techniques Assignment 7 out
16 Collagen structure and folding in vivo
17 Procollagen folding in vitro
18 Protein calorimetry: BPTI
19 Protein misfolding and aggregation
20

Ribosome channel

Nascent chains

Trigger factor

Graded problem set due
21 Scaffolding proteins in viral shell assembly
22 Amyloid fiber formation in neuro degenerative disease
23 Mid-term exam
24 Chaperonin assisted folding
25 Eukariotic chaperonins
26 Paper topic discussion and choices
27 Membrane protein (rhodopsin) folding and assembly
28 Prion diseases
29 Etiology of some human protein deposition diseases
30-37 Student presentations
38 Final papers due